S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest.

نویسندگان

  • Peng Zhao
  • Juan Ling Fu
  • Bi Yun Yao
  • Yong Rui Jia
  • Zong Can Zhou
چکیده

In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) incorporation rate, was introduced to detect S arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, SFN-BrdU incorporation rate can be used to detecting S arrest.

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عنوان ژورنال:
  • Biomedical and environmental sciences : BES

دوره 27 3  شماره 

صفحات  -

تاریخ انتشار 2014